As the pcr reaction proceeds at each round of amplification sybr green dye binds to dsdna as it polymerizes resulting in an increase in the level of fluorescence at the end of each extension step. Fastreal time pcr results in as fast as 35 minutes sensit. Experience specificity be confident using the chemically modified faststart taq dna polymerase even when your complete reaction mix sits for several hours at room temperature when using for example robotic setup.
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